Uteroferrin contains complex and high mannose-type oligosaccharides when synthesized in vitro. Academic Article

abstract

• Mature uteroferrin (Uf; Mr = 35,500) is a progesterone-induced acid phosphatase secreted by the pig uterus. It contains a single, unphosphorylated, high mannose-type oligosaccharide. Endometrial explants cultured in vitro secrete Uf with a Mr of 37,000 (37k Uf) having phosphorylated high mannose oligosaccharides. In this report we demonstrate that 37k Uf contains two N-linked oligosaccharides which are a mixture of complex and high mannose-type oligosaccharides. The complex-type glycopeptides are biantennary and a portion may be fucosylated on the GlcNac of the chitobiose core proximal to the peptide. Only a portion of the high mannose-type oligosaccharides are phosphorylated. The remainder appear to be typical Man6-4GlcNac2 oligosaccharides found on mature Uf.

authors

• Bazer, Fuller

published proceedings

• Mol Cell Biochem

altmetric score

• 3

author list (cited authors)

• Baumbach, G. A., Saunders, P. T., Ketcham, C. M., Bazer, F. W., & Roberts, R. M.

citation count

• 20

complete list of authors

• Baumbach, GA||Saunders, PT||Ketcham, CM||Bazer, FW||Roberts, RM

publication date

• July 1991

publisher

• Springer Nature  Publisher

published in

• MOLECULAR AND CELLULAR BIOCHEMISTRY  Journal

keywords

• Acid Phosphatase
• Amino Acid Sequence
• Animals
• Carbohydrate Sequence
• Chromatography, Affinity
• Concanavalin A
• Culture Techniques
• Electrophoresis, Polyacrylamide Gel
• Endometrium
• Female
• Glycosylation
• Hexosaminidases
• Isoenzymes
• Mannose
• Metalloproteins
• Molecular Sequence Data
• Molecular Weight
• Oligosaccharides
• Phosphorylation
• Precipitin Tests
• Swine
• Tartrate-resistant Acid Phosphatase

PubMed Central ID

• 1922010

Digital Object Identifier (DOI)

• 10.1007/BF00227750

start page

• 107

end page

• 117

volume

• 105

issue

• 2

URL

• http://dx.doi.org/10.1007/bf00227750