Nanofluidic devices for single molecule identification Academic Article

abstract

• In this letter, detection of target protein molecules has been demonstrated by a two-dimensional (2D) photon burst analysis technique and microfluidic channel device. Individual molecule events are scatter plotted in terms of their photon counts and burst width in 2D photon burst analysis. Detection of target protein is verified by a higher counts-per-bin for rabbit anti-HA polyclonal antibody sample than that of the control sample from the 2D photon burst analysis. Since this is microfluidic single molecule detection scheme, it has the advantages of high sensitivity, small sample consumption and reduced processing time, comparing to the conventional immunological ensemble measurements, such as western blot, immuno-precipitation, etc. 2008 CPST.

authors

• Kameoka, Jun

published proceedings

• JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY

author list (cited authors)

• Jing, N., Kameoka, J., Su, C. B., Chou, C., & Hung, M.

citation count

• 1

complete list of authors

• Jing, Nan||Kameoka, Jun||Su, Chin B||Chou, Chao-Kai||Hung, Mien-Chie

publication date

• September 2008

publisher

• Technical Association of Photopolymers, Japan  Publisher

published in

• Journal of photopolymer science and technology = [Fotoporima Konwakai shi]  Journal

keywords

• Microfluidic
• Quantum Dot
• Single Molecule Detection

Digital Object Identifier (DOI)

• 10.2494/photopolymer.21.531

start page

• 531

end page

• 536

volume

• 21

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.2494%2Fphotopolymer.21.531