Determination of protein concentration with two-dimensional (2D) Photon burst diagrams using microfluidic channel Conference Paper uri icon

abstract

  • The determination of target protein concentration has been demonstrated by a 2D photon burst (photon counts vs. burst width) analysis technique and microfluidic channel device. Since this technique is based on counting individual molecule events, we can effectively estimate the concentration of the specific target molecule among other molecules in a sample solution in a simpler, faster and more accurate way comparing to the conventional ensemble measurement techniques. It is also possible to study protein-protein interaction with only one fluorescent label. This could find potential applications in clinical diagnostics and signal pathway study in the future. 2008 CBMS.

published proceedings

  • 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

author list (cited authors)

  • Jing, N., Su, C. B., Chou, C. K., Hung, M. C., & Kameoka, J.

complete list of authors

  • Jing, N||Su, CB||Chou, CK||Hung, MC||Kameoka, J

publication date

  • January 2008