A polymeric capillary electrophoretic microfluidic device for separation and electrospray ionization of small molecules Conference Paper uri icon

abstract

  • 2001 Japan Soc. Of Applied Physics. We have used an embossed plastic microfluidic system for rapid electrophoretic separation of small molecules and electrospray ionization. We have also visualized the separation of compounds by observation of dyes in microfluidic systems and electrospray from the edge of the device. We used a lithographically produced silicon master to emboss channels in ZEONOR 1020R plastic. An oxygen plasma or chromic acid was used to convert the plastic channel surface from hydrophobic to hydrophilic for the separation of molecules in aqueous solution. The channel was scaled by thermal bonding of a cover piece of the same plastic material. The microfluidic device for electrophoretic separation and fluorescence detection had a separation channel 50 m wide, 20 m deep and 3.0 cm long. This was used to separate two dye molecules, Rhodamine B and Texas Red. The microfluidic device for electrospray ionization directly from the chip had a separation channel 30 m wide, 10 m deep, and 10 m wide, terminating in a nozzle-like pyramid fabricated at the edge of device. The sample volume injected through the cross junction for fluorescence detection device was approximately 1.2 nl and two dyes were separated within 8 seconds with the application of an electric field 300 V/cm.

name of conference

  • Digest of Papers. Microprocesses and Nanotechnology 2001. 2001 International Microprocesses and Nanotechnology Conference (IEEE Cat. No.01EX468)

published proceedings

  • MICROPROCESSES AND NANOTECHNOLOGY 2001, DIGEST OF PAPERS

author list (cited authors)

  • Kameoka, J., Zhong, H. W., Henion, J., Mawhinney, D. B., & Craighead, H. G.

citation count

  • 0

complete list of authors

  • Kameoka, J||Zhong, HW||Henion, J||Mawhinney, DB||Craighead, HG

publication date

  • January 2001