Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation Conference Paper uri icon

abstract

  • 2002 IEEE. Antibodies (Ab) are patterned at nanoscale precision for the precise immobilization and stimulation of immune cells. We demonstrate that the antigen bovine serum albumin (BSA) can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one contiguous piece in aqueous solution. Anti-BSA Ab are bound specifically to BSA to create a pattern of oriented Ab that provides a surface for eosinophil immobilization and degranulation. The patterns ranged from 0.36 m2 to 4,489 m2, appropriate dimensions for the 10-14 m diameter eosinophil cells. This method provides a new technique for immobilizing cells onto nano and micrometer scale patterns for analyzing cellular biochemical cascade events such as degranulation and studying cellular morphological changes in response to defined nanoscale antigenic stimulus.

name of conference

  • 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.02EX578)

published proceedings

  • 2ND ANNUAL INTERNATIONAL IEEE-EMBS SPECIAL TOPIC CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE & BIOLOGY, PROCEEDINGS

author list (cited authors)

  • Orth, R. N., Kwan, L., Kameoka, J., Lindau, M., & Craighead, H. G.

citation count

  • 0

complete list of authors

  • Orth, RN||Kwan, L||Kameoka, J||Lindau, M||Craighead, HG

publication date

  • January 2002