Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution. Academic Article uri icon


  • The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid-phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra-day assay precision of 2% to 15% and an inter-day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds.

published proceedings

  • Rapid Commun Mass Spectrom

author list (cited authors)

  • Li, W., Herrman, T. J., & Dai, S. Y.

citation count

  • 22

complete list of authors

  • Li, Wei||Herrman, Timothy J||Dai, Susie Y

publication date

  • May 2011