Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution.

abstract

• The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid-phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra-day assay precision of 2% to 15% and an inter-day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds.

authors

• Dai, Susie
• Herrman, Timothy

published proceedings

• Rapid Commun Mass Spectrom

author list (cited authors)

• Li, W., Herrman, T. J., & Dai, S. Y.

citation count

• 22

complete list of authors

• Li, Wei||Herrman, Timothy J||Dai, Susie Y

publication date

• May 2011

publisher

• Wiley  Publisher

published in

• Rapid Communications in Mass Spectrometry  Journal

keywords

• Aflatoxins
• Animal Feed
• Chromatography, Liquid
• Isotope Labeling
• Reproducibility Of Results
• Sensitivity And Specificity
• Solid Phase Extraction
• Tandem Mass Spectrometry

PubMed Central ID

• 21491528

Digital Object Identifier (DOI)

• 10.1002/rcm.4979

start page

• 1222

end page

• 1230

volume

• 25

issue

• 9

URL

• http%3A%2F%2Fdx.doi.org%2F10.1002%2Frcm.4979