Synthesis and secretion of tobacco neutral PR-5 protein by transgenic tobacco and yeast. Academic Article uri icon


  • Tobacco neutral osmotin-like protein was expressed in both homologous and heterologous expression systems. Osmotin-like protein was retained in tobacco cells when expressed as the prepro-form, whereas deletion of the carboxyl-terminal propeptide of 22 amino acids induced its secretion into the extracellular fluid. Without the amino-terminal signal peptide, there was no accumulation of osmotin-like protein. When the construct with both the amino- and carboxyl-terminal peptides was introduced to methylotrophic yeast, divergent osmotin-like protein forms were found in the cell and medium. Results suggest that the amino-terminal signal of the osmotin-like protein is essential for the transport of protein to the endoplasmic reticulum both in tobacco and yeasts but that the carboxyl-terminal propeptide signal is effective only for intracellular retention in tobacco.

published proceedings

  • Biochem Biophys Res Commun

altmetric score

  • 6

author list (cited authors)

  • Sato, F., Koiwa, H., Sakai, Y., Kato, N., & Yamada, Y.

citation count

  • 25

complete list of authors

  • Sato, F||Koiwa, H||Sakai, Y||Kato, N||Yamada, Y

publication date

  • January 1995