The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors. Academic Article

abstract

• Proteins in CPL1 family are unique to plants and contain a phosphatase catalytic domain and double-stranded RNA (dsRNA)-binding motifs (DRMs) in a single peptide. Though DRMs are important for the function of Arabidopsis CPL1 in vivo, the role of CPL1 DRM has been obscure. We have isolated two transcription factors, ANAC019 (At1g52890) and AtMYB3 (At1g22640), which specifically interact with the C-terminal region (640-967) of AtCPL1 containing two DRMs. Detailed interaction analysis indicated that AtMYB3 specifically interacted with the first DRM but not with the second DRM in CPL1 C-terminal fragment. GFP-fusion analysis indicated that AtMYB3 localized in nuclei-like CPL1, and its expression is induced by abiotic stress and ABA treatment. These results suggest that AtMYB3 function in abiotic stress signaling in concert with CPL1.

authors

• Koiwa, Hisashi

published proceedings

• Biochem Biophys Res Commun

author list (cited authors)

• Bang, W. Y., Kim, S. W., Jeong, I. S., Koiwa, H., & Bahk, J. D.

citation count

• 18

complete list of authors

• Bang, Woo Young||Kim, Se Won||Jeong, In Sil||Koiwa, Hisashi||Bahk, Jeong Dong

publication date

• January 2008

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Amino Acid Motifs
• Arabidopsis Proteins
• Cell Nucleus
• Environment
• Green Fluorescent Proteins
• Phosphoprotein Phosphatases
• RNA, Double-Stranded
• RNA-Binding Proteins
• Transcription Factors
• Two-Hybrid System Techniques

Digital Object Identifier (DOI)

• 10.1016/j.bbrc.2008.05.161

start page

• 907

end page

• 912

volume

• 372

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.bbrc.2008.05.161