One-step casting of Laemmli discontinued sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel. Academic Article

abstract

• A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can be cast at the same time because the lower gel contains 10% glycerol, which generates higher density in the lower gel than in the upper gel.

authors

• Koiwa, Hisashi

published proceedings

• Anal Biochem

author list (cited authors)

• Wu, X., & Koiwa, H.

citation count

• 6

complete list of authors

• Wu, Xiaoqiang||Koiwa, Hisashi

publication date

• January 2012

publisher

• Elsevier  Publisher

published in

• Analytical Biochemistry  Journal

keywords

• Electrophoresis, Polyacrylamide Gel
• Escherichia Coli Proteins
• Gels
• Sodium Dodecyl Sulfate
• Time Factors
• Tromethamine

Digital Object Identifier (DOI)

• 10.1016/j.ab.2011.10.004

start page

• 347

end page

• 349

volume

• 421

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.ab.2011.10.004