One-step casting of Laemmli discontinued sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel. Academic Article uri icon

abstract

  • A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can be cast at the same time because the lower gel contains 10% glycerol, which generates higher density in the lower gel than in the upper gel.

published proceedings

  • Anal Biochem

author list (cited authors)

  • Wu, X., & Koiwa, H.

citation count

  • 6

complete list of authors

  • Wu, Xiaoqiang||Koiwa, Hisashi

publication date

  • February 2012