Specific control of Arabidopsis BAK1/SERK4-regulated cell death by protein glycosylation.
- Additional Document Info
- View All
Precise control of cell death is essential for the survival of all organisms. Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1) and somatic embryogenesis receptor kinase 4 (SERK4) redundantly and negatively regulate cell death through elusive mechanisms. By deploying a genetic screen for suppressors of cell death triggered by virus-induced gene silencing of BAK1/SERK4 on Arabidopsis knockout collections, we identified STT3a, a protein involved in N-glycosylation modification, as an important regulator of bak1/serk4 cell death. Systematic investigation of glycosylation pathway and endoplasmic reticulum (ER) quality control (ERQC) components revealed distinct and overlapping mechanisms of cell death regulated by BAK1/SERK4 and their interacting protein BIR1. Genome-wide transcriptional analysis revealed the activation of members of cysteine-rich receptor-like kinase (CRK) genes in the bak1/serk4 mutant. Ectopic expression of CRK4 induced STT3a/N-glycosylation-dependent cell death in Arabidopsis and Nicotiana benthamiana. Therefore, N-glycosylation and specific ERQC components are essential to activate bak1/serk4 cell death, and CRK4 is likely to be among client proteins of protein glycosylation involved in BAK1/SERK4-regulated cell death.
author list (cited authors)
de Oliveira, M., Xu, G., Li, B. o., de Souza Vespoli, L., Meng, X., Chen, X., ... He, P.
complete list of authors
de Oliveira, Marcos VV||Xu, Guangyuan||Li, Bo||de Souza Vespoli, Luciano||Meng, Xiangzong||Chen, Xin||Yu, Xiao||de Souza, Suzane Ariádina||Intorne, Aline C||de A Manhães, Ana Marcia E||Musinsky, Abbey L||Koiwa, Hisashi||de Souza Filho, Gonçalo A||Shan, Libo||He, Ping