An ATP analog-sensitive version of the tomato cell death suppressor protein kinase Adi3 for use in substrate identification. Academic Article

abstract

• Adi3 is a protein kinase from tomato that functions as a cell death suppressor and its substrates are not well defined. As a step toward identifying Adi3 substrates we developed an ATP analog-sensitive version of Adi3 in which the ATP-binding pocket is mutated to allow use of bulky ATP analogs. Met385 was identified as the "gatekeeper" residue and the M385G mutation allows for the use of two bulky ATP analogs. Adi3(M385G) can also specifically utilize N(6)-benzyl-ATP to phosphorylate a known substrate and provides a tool for identifying Adi3 substrates.

authors

• Devarenne, Timothy

published proceedings

• Biochim Biophys Acta

author list (cited authors)

• Dittrich, A., & Devarenne, T. P.

citation count

• 3

complete list of authors

• Dittrich, Anna C Nelson||Devarenne, Timothy P

publication date

• February 2012

publisher

• Elsevier  Publisher

published in

• Biochimica et Biophysica Acta: international journal of biochemistry and biophysics  Journal

keywords

• Adenosine Triphosphate
• Amino Acid Sequence
• Apoptosis
• Binding Sites
• Cloning, Molecular
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Phosphorylation
• Plant Proteins
• Protein Serine-threonine Kinases
• Solanum Lycopersicum
• Substrate Specificity

PubMed Central ID

• 22027266

Digital Object Identifier (DOI)

• 10.1016/j.bbapap.2011.10.004

start page

• 269

end page

• 273

volume

• 1824

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.bbapap.2011.10.004