Mono-(2-ethylhexyl) Phthalate Increases Oxidative Stress Responsive miRNAs in First Trimester Placental Cell Line HTR8/SVneo. Academic Article

abstract

• Phthalates, an endocrine disruptor group, cause oxidative stress (OS) in the placenta. However, no studies have reported OS-related miRNAs induced by phthalates. In the present study, we demonstrate that mono-(2-ethylhexyl) phthalate (MEHP) induces OS responsive miR-17-5p, miR-155-5p, and miR-126-3p in HTR8/SVneo in a dose- and time-dependent manner. Furthermore, MEHP altered the expression of phosphoinositide-3-kinase regulatory subunit 1, phosphatase and tensin homolog, CDKN2A interacting protein, superoxide dismutase 2, and 3-hydroxysterol-D24 reductase, which are involved in OS and predicted to be regulated by these miRNAs. Our results suggest that placental exposure to MEHP may result in aberrant miRNA expression leading to pregnancy complications.

authors

• Choudhury, Mahua

published proceedings

• Chem Res Toxicol

altmetric score

• 0.25

author list (cited authors)

• Meruvu, S., Zhang, J., & Choudhury, M.

citation count

• 42

complete list of authors

• Meruvu, Sunitha||Zhang, Jian||Choudhury, Mahua

publication date

• March 2016

publisher

• American Chemical Society (ACS)  Publisher

published in

• CHEMICAL RESEARCH IN TOXICOLOGY  Journal

keywords

• Cell Survival
• Cells, Cultured
• Diethylhexyl Phthalate
• Dose-Response Relationship, Drug
• Female
• Humans
• MicroRNAs
• Molecular Structure
• Oxidative Stress
• Pregnancy
• Pregnancy Trimester, First
• Reactive Oxygen Species
• Structure-Activity Relationship
• Time Factors

PubMed Central ID

• 26871967

Digital Object Identifier (DOI)

• 10.1021/acs.chemrestox.6b00038

start page

• 430

end page

• 435

volume

• 29

issue

• 3

URL

• http://dx.doi.org/10.1021/acs.chemrestox.6b00038

user-defined tag

• 3 Good Health and Well-Being