Polylysine enhances cationic liposome-mediated transfection of the hepatoblastoma cell line Hep G2.

abstract

• Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly-L-lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2-dioleoyloxy-3-trimethyl-ammoniumpropane, with the neutral phospholipid 1,2-di-(cis-9-octadecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced cationic-liposome-mediated transfection of the hepatoblastoma cell line Hep G2 9-fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine-pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells.

authors

• Walzem, Rosemary

published proceedings

• Biotechnol Appl Biochem

author list (cited authors)

• Mack, K. D., Walzem, R. L., Lehmann-Bruinsma, K., Powell, J. S., & Zeldis, J. B.

citation count

• 8

complete list of authors

• Mack, KD||Walzem, RL||Lehmann-Bruinsma, K||Powell, JS||Zeldis, JB

publication date

• June 1996

published in

• BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY  Journal

keywords

• Cations
• Electrophoresis, Agar Gel
• Fatty Acids, Monounsaturated
• Hepatoblastoma
• Humans
• Liposomes
• Liver Neoplasms
• Luciferases
• Particle Size
• Phosphatidylethanolamines
• Plasmids
• Polylysine
• Quaternary Ammonium Compounds
• Transfection
• Tumor Cells, Cultured

PubMed Central ID

• 8679107

Digital Object Identifier (DOI)

• 10.1111/j.1470-8744.1996.tb00378.x

start page

• 217

end page

• 220

volume

• 23

issue

• 3