Cholesterol intake modulates plasma triglyceride levels in glycosylphosphatidylinositol HDL-binding protein 1-deficient mice. Academic Article uri icon

abstract

  • OBJECTIVE: To determine whether plasma triglyceride levels in adult Glycosylphosphatidylinositol HDL-binding protein 1 (GPIHBP1)-deficient (Gpihbp1(-/-)) mice would be sensitive to cholesterol intake. METHODS AND RESULTS: Gpihbp1(-/-) mice were fed a Western diet containing 0.15% cholesterol. After 4 to 8 weeks, their plasma triglyceride levels were 113 to 135 mmol/L. When 0.005% ezetimibe was added to the diet to block cholesterol absorption, Lpl expression in the liver was reduced significantly, and the plasma triglyceride levels were significantly higher (>170 mmol/L). We also assessed plasma triglyceride levels in Gpihbp1(-/-) mice fed Western diets containing either high (1.3%) or low (0.05%) amounts of cholesterol. The high-cholesterol diet significantly increased Lpl expression in the liver and lowered plasma triglyceride levels. CONCLUSIONS: Treatment of Gpihbp1(-/-) mice with ezetimibe lowers Lpl expression in the liver and increases plasma triglyceride levels. A high-cholesterol diet had the opposite effects. Thus, cholesterol intake modulates plasma triglyceride levels in Gpihbp1(-/-) mice.

published proceedings

  • Arterioscler Thromb Vasc Biol

author list (cited authors)

  • Weinstein, M. M., Tu, Y., Beigneux, A. P., Davies, B., Gin, P., Voss, C., ... Young, S. G.

citation count

  • 15

complete list of authors

  • Weinstein, Michael M||Tu, Yiping||Beigneux, Anne P||Davies, Brandon SJ||Gin, Peter||Voss, Constance||Walzem, Rosemary L||Reue, Karen||Tontonoz, Peter||Bensadoun, AndrĂ©||Fong, Loren G||Young, Stephen G

publication date

  • November 2010