Quantitative characterization of aqueous solutions probed by the third-harmonic generation microscopy. Academic Article

abstract

• Third-harmonic microscopy is one of the emerging techniques for noninvasive microscopic imaging of biological structures. We use a novel technique for nonlinear optical material characterization and study the effect of different environment and the structural sensitivity of the third harmonic. In particular, a transformation of collagen in solution is observed for the first time using third-harmonic generation. We also study the ultimate limits of the third harmonic to detect micro- and nanoscopic features inside living cells and find that structures as small as 50 nm can be detected using the current level of technology.

authors

• Yakovlev, Vladislav

published proceedings

• J Struct Biol

author list (cited authors)

• Shcheslavskiy, V., Petrov, G. I., Saltiel, S., & Yakovlev, V. V.

citation count

• 21

complete list of authors

• Shcheslavskiy, V||Petrov, GI||Saltiel, S||Yakovlev, VV

publication date

• July 2004

publisher

• Elsevier  Publisher

published in

• Journal of Structural Biology  Journal

keywords

• Collagen
• Imaging, Three-Dimensional
• Lasers
• Mathematics
• Microscopy
• Particle Size
• Solutions
• Water

PubMed Central ID

• 15109604

Digital Object Identifier (DOI)

• 10.1016/j.jsb.2003.10.015

start page

• 42

end page

• 49

volume

• 147

issue

• 1