Quantitative characterization of aqueous solutions probed by the third-harmonic generation microscopy. Academic Article uri icon


  • Third-harmonic microscopy is one of the emerging techniques for noninvasive microscopic imaging of biological structures. We use a novel technique for nonlinear optical material characterization and study the effect of different environment and the structural sensitivity of the third harmonic. In particular, a transformation of collagen in solution is observed for the first time using third-harmonic generation. We also study the ultimate limits of the third harmonic to detect micro- and nanoscopic features inside living cells and find that structures as small as 50 nm can be detected using the current level of technology.

published proceedings

  • J Struct Biol

author list (cited authors)

  • Shcheslavskiy, V., Petrov, G. I., Saltiel, S., & Yakovlev, V. V.

citation count

  • 21

complete list of authors

  • Shcheslavskiy, V||Petrov, GI||Saltiel, S||Yakovlev, VV

publication date

  • July 2004