Analytical Capabilities of Coherent Anti-Stokes Raman Scattering Microspectroscopy.
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Nonlinear Raman scattering is an emerging spectroscopy technique for non-invasive microscopic imaging. It can produce a fluorescence background free vibrational spectrum from a microscopic volume of a sample providing chemically specific information about its molecular composition. We analyze the ability of nonlinear Raman microspectroscopy to detect low concentrated molecular species and evaluate its applicability to study complex solutions.