Raman spectroscopy compatible PDMS droplet microfluidic culture and analysis platform towards on-chip lipidomics Academic Article uri icon

abstract

  • Lipids produced by microalgae are viewed as a potential renewable alternative to fossil fuels, however, significant improvements in productivity are required for microalgal biofuels to become economically feasible. Here we present a method that allows for the use of Raman spectroscopy with poly(dimethylsiloxane) (PDMS) droplet microfluidic devices, which not only overcomes the high Raman background of PDMS, but also achieves pairing of the high-throughput single-cell resolution advantages of droplet microfluidics with the direct, chemically specific, label-free, and non-destructive nature of Raman spectroscopy. The platform was successfully utilized for in situ characterization of microalgal lipid production over time within droplets, paving the way towards high-throughput microalgal lipidomics assays.

altmetric score

  • 3

author list (cited authors)

  • Kim, H. S., Waqued, S. C., Nodurft, D. T., Devarenne, T. P., Yakovlev, V. V., & Han, A.

citation count

  • 24

publication date

  • April 2017