The role of an extracellular chitinase from Trichoderma virens Gv29-8 in the biocontrol of Rhizoctonia solani. Academic Article

abstract

• The role of extracellular chitinase in the biocontrol activity of Trichoderma virens was examined using genetically manipulated strains of this fungus. The T. virens strains in which the chitinase gene (cht42) was disrupted (KO) or constitutively over-expressed (COE) were constructed through genetic transformation. The resulting transformants were stable and showed patterns similar to the wild-type (WT) strain with respect to growth rate, sporulation, antibiotic production, colonization efficiency on cotton roots and growth/survival in soil. Biocontrol activity of the KO and COE strains were significantly decreased and enhanced, respectively against cotton seedling disease incited by Rhizoctonia solani when compared with the WT strain.

authors

• Kenerley, Charles

published proceedings

• Curr Genet

author list (cited authors)

• Baek, J. M., Howell, C. R., & Kenerley, C. M.

citation count

• 126

complete list of authors

• Baek, JM||Howell, CR||Kenerley, CM

publication date

• January 1999

publisher

• Springer Nature  Publisher

published in

• Current Genetics  Journal

keywords

• Cell Division
• Chitinases
• Cloning, Molecular
• Fungal Proteins
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Fungal
• Gene Targeting
• Genes, Fungal
• Pest Control, Biological
• Plant Diseases
• RNA, Messenger
• Rhizoctonia
• Sequence Homology, Amino Acid
• Transformation, Genetic
• Trichoderma

Digital Object Identifier (DOI)

• 10.1007/s002940050431

start page

• 41

end page

• 50

volume

• 35

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2Fs002940050431