Elution of macrophage-bound immunoglobulins by temperature changes in vitro. Academic Article

abstract

• Some mouse anti-sheep-erythrocyte macrophage cytophilic antibodies are more readily adsorbed to cells at 4 than at 37. By treating cells with serum at 4, washing them well and eluting bound material at 30, it was found possible to isolate cytophilic immunoglobulins. These were found to have fast -mobility, but were heterogeneous upon preparative ultracentrifugation and gel-filtration. The macrophage eluate contained relatively high titres of anti-sheep-erythrocyte cytophilic antibodies.

authors

• Tizard, Ian

published proceedings

• Immunology

author list (cited authors)

• Tizard, I. R.

citation count

• 9

complete list of authors

• Tizard, IR

publication date

• January 1972

published in

• Immunology  Journal

keywords

• Adsorption
• Animals
• Antibodies
• Centrifugation, Density Gradient
• Erythrocytes
• Hemagglutination Tests
• Hemolysis
• Immunoelectrophoresis
• Immunoglobulins
• Macrophages
• Mice
• Opsonin Proteins
• Peritoneum
• Sheep
• Temperature

PubMed Central ID

• 5013911

start page

• 69

end page

• 73

volume

• 22

issue

• 1