Isolation of phospholipase A1 from Trypanosoma brucei. Academic Article

abstract

• Phospholipase A1 from Trypanosoma brucei brucei has been purified 380-fold by column chromatography on phosphatidylcholine-Sepharose affinity columns followed by DEAE-cellulose anion-exchange chromatography and Sephacryl S-200 molecular exclusion chromatography. Octyl-Agarose hydrophobic column chromatography can be substituted for the PC-Sepharose column. The molecular weight of trypanosomal PLase A1 was found to be 26,000 by SDS-polyacrylamide gel electrophoresis.

authors

• Tizard, Ian

published proceedings

• Tropenmed Parasitol

author list (cited authors)

• Hambrey, P. N., Forsberg, C. M., Mellors, A., Tizard, I. R., & Werchola, G. M.

citation count

• 13

complete list of authors

• Hambrey, PN||Forsberg, CM||Mellors, A||Tizard, IR||Werchola, GM

publication date

• March 1984

published in

• Tropenmedizin und Parasitologie  Journal

keywords

• Animals
• Chromatography, Affinity
• Chromatography, DEAE-Cellulose
• Chromatography, Gel
• Electrophoresis, Polyacrylamide Gel
• Molecular Weight
• Phospholipases
• Phospholipases A
• Phospholipases A1
• Trypanosoma Brucei Brucei

PubMed Central ID

• 6710597

start page

• 15

end page

• 19

volume

• 35

issue

• 1