Crystal structure of shrimp arginine kinase in binary complex with arginine-a molecular view of the phosphagen precursor binding to the enzyme.
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abstract
Arginine kinase (AK) is a key enzyme for energetic balance in invertebrates. Although AK is a well-studied system that provides fast energy to invertebrates using the phosphagen phospho-arginine, the structural details on the AK-arginine binary complex interaction remain unclear. Herein, we determined two crystal structures of the Pacific whiteleg shrimp (Litopenaeus vannamei) arginine kinase, one in binary complex with arginine (LvAK-Arg) and a ternary transition state analog complex (TSAC). We found that the arginine guanidinium group makes ionic contacts with Glu225, Cys271 and a network of ordered water molecules. On the zwitterionic side of the amino acid, the backbone amide nitrogens of Gly64 and Val65 coordinate the arginine carboxylate. Glu314, one of proposed acid-base catalytic residues, did not interact with arginine in the binary complex. This residue is located in the flexible loop 310-320 that covers the active site and only stabilizes in the LvAK-TSAC. This is the first binary complex crystal structure of a guanidine kinase in complex with the guanidine substrate and could give insights into the nature of the early steps of phosphagen biosynthesis.
Lpez-Zavala, A. A., Garca-Orozco, K. D., Carrasco-Miranda, J. S., Sugich-Miranda, R., Velzquez-Contreras, E. F., Criscitiello, M. F., ... Sotelo-Mundo, R. R.
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López-Zavala, Alonso A||García-Orozco, Karina D||Carrasco-Miranda, Jesús S||Sugich-Miranda, Rocio||Velázquez-Contreras, Enrique F||Criscitiello, Michael F||Brieba, Luis G||Rudiño-Piñera, Enrique||Sotelo-Mundo, Rogerio R