Differential Staining of Bacteria: Gram Stain Academic Article

abstract

• In 1884, Hans Christian Gram, a Danish doctor, developed a differential staining technique that is still the cornerstone of bacterial identification and taxonomic division. This multistep, sequential staining protocol separates bacteria into four groups based on cell morphology and cell wall structure: Gram-positive cocci, Gram-negative cocci, Gram-positive rods, and Gram-negative rods. The Gram stain is useful for assessing bacterial contamination of tissue culture samples or for examining the Gram stain status and morphological features of bacteria isolated from mixed or isolated bacterial cultures.

authors

• Moyes, Rita

altmetric score

• 1.85

author list (cited authors)

• Moyes, R. B., Reynolds, J., & Breakwell, D. P.

citation count

• 28

complete list of authors

• Moyes, Rita B||Reynolds, Jackie||Breakwell, Donald P

publication date

• November 2009

publisher

• Wiley  Publisher

published in

• Current protocols in microbiology  Journal

keywords

• Bacteria
• Bacterial Typing Techniques
• Coloring Agents
• Staining And Labeling

PubMed Central ID

• 19885931

Digital Object Identifier (DOI)

• 10.1002/9780471729259.mca03cs15

start page

• a.3c.1

end page

• a.3c.8

volume

• 15

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1002%2F9780471729259.mca03cs15