Combining AFM and FRET for studies at the cellular level Conference Paper uri icon


  • We report on the development of a Frster resonance energy transfer (FRET) enabled atomic force microscope (AFM) for the study of biomechanics and mechanobiology at the cellular level. The hybrid microscopy system combines the spatial resolution and control of the AFM with the nanoscale sensing capabilities of FRET to enable simultaneous detection of cell mechanical responses and correlation of those responses with cellular biochemistry. Here, we show FRET signal from donor-coated microspheres, that are attached to an AFM cantilever, to acceptor-labeled integrins in a fixed cell system. Additionally, we demonstrate and discuss the attachment of quantum dots to silica microspheres as the FRET donor.

name of conference

  • Optical Diagnostics and Sensing VIII

published proceedings


author list (cited authors)

  • Meissner, K. E., Sun, Z., Nathwani, B., Needham, C., Beckham, R. E., Everett, W. N., ... Meininger, G. A.

citation count

  • 1

complete list of authors

  • Meissner, Kenith E||Sun, Zhe||Nathwani, Bhavik||Needham, Clark||Beckham, Richard E||Everett, William N||Fan, Xudong||Cote, Gerard L||Meininger, Gerald A

editor list (cited editors)

  • Coté, G. L., & Priezzhev, A. V.

publication date

  • January 2008