Fiber-optic electron-spin-resonance thermometry of single laser-activated neurons. Academic Article

abstract

• Optically detected electron spin resonance in fiber-coupled nitrogen-vacancy (NV) centers of diamond is used to demonstrate a fiber-optic quantum thermometry of individual thermogenetically activated neurons. Laser-induced temperature variations read out from single neurons with the NV-diamond fiber sensor are shown to strongly correlate with the fluorescence of calcium-ion sensors, serving as online indicators of the inward Ca2+ current across the cell membrane of neurons expressing transient receptor potential (TRP) cation channels. Local laser heating above the TRP-channel activation threshold is shown to reproducibly evoke robust action potentials, visualized by calcium-ion-sensor-aided fluorescence imaging and detected as prominent characteristic waveforms in the time-resolved response of fluorescence Ca2+ sensors.

authors

• Hemmer, Philip

published proceedings

• Opt Lett

author list (cited authors)

• Lanin, A. A., Fedotov, I. V., Ermakova, Y. G., Sidorov-Biryukov, D. A., Fedotov, A. B., Hemmer, P., Belousov, V. V., & Zheltikov, A. M.

citation count

• 26

complete list of authors

• Lanin, AA||Fedotov, IV||Ermakova, Yu G||Sidorov-Biryukov, DA||Fedotov, AB||Hemmer, P||Belousov, VV||Zheltikov, AM

publication date

• December 2016

publisher

• Optica Publishing Group  Publisher

published in

• Optics Letters  Journal

keywords

• Diamond
• Electrons
• FLUORESCENCE
• Fiber Optic Technology
• Lasers
• Neurons
• Nitrogen
• Optical Fibers
• Temperature
• Thermometry

PubMed Central ID

• 27906239

Digital Object Identifier (DOI)

• 10.1364/OL.41.005563

start page

• 5563

end page

• 5566

volume

• 41

issue

• 23