De novo expression of human leukocyte antigen-DR (HLA-DR) and loss of beta-catenin expression in tubular epithelial cells: a possible event in epithelial-mesenchymal transition in canine renal diseases. Academic Article uri icon

abstract

  • Tubulointerstitial fibrosis (TIF) plays a central role in the progression to end-stage renal disease. Tubular epithelial cells (TECs) undergo epithelial-mesenchymal transition (EMT) and may contribute to the progression of TIF. Using immunohistochemistry, the primary aim of this study was to assess the expression of -catenin, human leukocyte antigen-DR (HLA-DR) and vimentin in renal biopsies from dogs with spontaneous kidney diseases of varying severities. Morphological diagnosis, severity of inflammation, TIF, HLA-DR expression and clinicopathological variables were compared in dogs with renal injury to identify any potential relationship between the different factors; -catenin down-regulation was used as a marker of EMT. Fibrosis, HLA-DR expression, serum creatinine concentration (SCr), and urine protein-to-creatinine ratio (UPC) were all increased and -catenin expression decreased in dogs with primary glomerular disease compared with dogs with acute tubular necrosis. HLA-DR expression by TECs was positively correlated to fibrosis, inflammation, UPC, and SCr. -catenin expression was negatively correlated to fibrosis, inflammation and HLA-DR expression. The progression of renal failure correlated closely with tubulointerstitial damage. De novo HLA-DR expression associated with -catenin down-regulation by TECs may represent a possible step in the progression of TIF and EMT.

published proceedings

  • Vet J

altmetric score

  • 0.25

author list (cited authors)

  • Benali, S. L., Lees, G. E., Nabity, M. B., Mantovani, R., Bonsembiante, F., & Aresu, L.

citation count

  • 3

complete list of authors

  • Benali, SL||Lees, GE||Nabity, MB||Mantovani, R||Bonsembiante, F||Aresu, L

publication date

  • January 2013