Murine Colonic Organoid Culture System and Downstream Assay Applications. Academic Article

abstract

• Colonic organoids, three-dimensional colonic crypts grown in vitro that show realistic microanatomy, have many potential applications for studying physiology, developmental biology, and pathophysiology of intestinal diseases including inflammatory bowel disease and colorectal cancer. Here, we describe detailed protocols for mouse colonic crypt isolation, organoid culture, and downstream applications. Specific culture strategies including growth factor enriched Matrigel and Wnt and R-spondin conditioned media serve as key factors for enhancing the growth and cost efficiency of colonic organoid cultures.

authors

• Chapkin, Robert

published proceedings

• Methods Mol Biol

altmetric score

• 1.35

author list (cited authors)

• Fan, Y., Davidson, L. A., & Chapkin, R. S.

citation count

• 15

complete list of authors

• Fan, Yang-Yi||Davidson, Laurie A||Chapkin, Robert S

publication date

• January 2019

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Cell Culture Techniques
• Cell Differentiation
• Cells, Cultured
• Colon
• Culture Media, Conditioned
• In Vitro Primary Culture
• Intercellular Signaling Peptides And Proteins
• Mice
• Niche-independent Crypt Expansion
• Organoids
• Stem Cells

PubMed Central ID

• 27539462

Digital Object Identifier (DOI)

• 10.1007/7651_2016_8

start page

• 171

end page

• 181

volume

• 1576

URL

• http://dx.doi.org/10.1007/7651_2016_8