Mass cloning of differential and nondifferential transcript-derived fragments from cDNA-AFLP experiments in sunflower Academic Article uri icon


  • We have developed a 1-step procedure for cloning multiple bands obtained through cDNA-amplified fragment length polymorphism (AFLP) experiments. This protocol enables us to clone differential and nondifferential transcript-derived fragments (TDFs) in the same experiment. It consists of a mass cloning of cDNA-AFLP amplification products, identification of clones of interest with electrophoresis, and characterization through sequencing. The entire experiment can be performed in 1 wk because of the reduced time and effort needed for cloning, and it can be applied to any PCR method that produces complex band patterns. This method, tested on a comparison of gene expression between globular and heart embryo stages in sunflower embryogenesis, enabled us to clone 10 differential TDFs in the same experiment. 2004 International Society for Plant Molecular Biology.

published proceedings


author list (cited authors)

  • Tamborindeguy, C., Ben, C., Jardinaud, F., Gentzbittel, L., & Liboz, T.

citation count

  • 3

complete list of authors

  • Tamborindeguy, C||Ben, C||Jardinaud, F||Gentzbittel, L||Liboz, T

publication date

  • June 2004