Mapping of bovine PRGS and PAIS genes in hybrid somatic cells: syntenic conservation with human chromosome 21. Academic Article

abstract

• Somatic cell genetics coupled with enzyme electrophoresis has facilitated the mapping of PRGS and PAIS genes in cattle. Individual cow-hamster hybrid cell lines established by fusion of mutant CHO cells, ade-C and ade-G, with cattle leukocytes required complementing bovine genes for PRGS and PAIS, respectively, when propogated on selective media. Homogenates of 12 PRGS+ hybrid clones and 12 PAIS+ hybrid clones retained the bovine electromorph of SOD1 while extensively segregating 14 biochemical markers of other cattle syntenic groups. Secondary cattle-hamster hybrid subclones which segregated bovine PRGS and PAIS in late passages on nonselective media concordantly segregated bovine SOD1. These data support a syntenic relationship among PRGS, PAIS, and SOD1 on cattle syntenic group U10. An interferon receptor locus, IFREC, is also syntenic with SOD1. This synteny represents an extensive conservation of bovine U10 and the "Down syndrome region" of human chromosome 21.

authors

• Womack, James

published proceedings

• Biochem Genet

author list (cited authors)

• McAvin, J. C., Patterson, D., & Womack, J. E.

citation count

• 9

complete list of authors

• McAvin, JC||Patterson, D||Womack, JE

publication date

• February 1988

publisher

• Springer Nature  Publisher

published in

• Biochemical Genetics  Journal

keywords

• Animals
• Carbon-nitrogen Ligases
• Cattle
• Cell Line
• Chromosome Mapping
• Chromosomes, Human, Pair 21
• Cricetinae
• Cricetulus
• Genes
• Humans
• Hybrid Cells
• Isoenzymes
• Ligases
• Mutation

Digital Object Identifier (DOI)

• 10.1007/BF00555485

start page

• 9

end page

• 18

volume

• 26

issue

• 1-2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2Fbf00555485