F-actin localization dynamics during appressorium formation in Colletotrichum graminicola. Academic Article

abstract

• Appressoria are essential penetration structures for many phytopathogenic fungi. Here F-actin localization dynamics were documented during appressorium formation in vitro and in planta in Colletotrichum graminicola Four discernible stages of dynamic F-actin distribution occurring in a programmed order were documented from differentiation of appressoria to formation of penetration pores: (stage A) from germ tube enlargement to complete expansion of the appressorium; (stage S) septation occurs; (stage L) a long period of low F-actin activity; (stage P) the penetration pore forms. The F-actin subcellular localization corresponded to each stage. A distinct redistribution of actin cables occurred at the transition from stage A to stage S. The in planta assays revealed that F-actin also assembled in invasive hyphae and that actin cables might play an essential role for penetration-peg development. The F-actin localization distribution may be used as a subcellular marker to define the developmental stages during appressorium formation.

authors

• Shaw, Brian

published proceedings

• Mycologia

author list (cited authors)

• Wang, C., & Shaw, B. D.

citation count

• 15

complete list of authors

• Wang, Chih-Li||Shaw, Brian D

publication date

• January 2016

publisher

• Taylor & Francis  Publisher

published in

• Mycologia  Journal

keywords

• Actin
• Actins
• Anthracnose
• Appressorium
• Colletotrichum
• Fungal Proteins
• Gene Expression Regulation, Fungal
• Lifeact
• Maize
• Penetration Peg
• Protein Transport
• Spores, Fungal

Digital Object Identifier (DOI)

• 10.3852/15-068

start page

• 506

end page

• 514

volume

• 108

issue

• 3

URL

• http%3A%2F%2Fdx.doi.org%2F10.3852%2F15-068