Lab-on-a-Drone: Toward Pinpoint Deployment of Smartphone-Enabled Nucleic Acid-Based Diagnostics for Mobile Health Care. Academic Article uri icon

abstract

  • We introduce a portable biochemical analysis platform for rapid field deployment of nucleic acid-based diagnostics using consumer-class quadcopter drones. This approach exploits the ability to isothermally perform the polymerase chain reaction (PCR) with a single heater, enabling the system to be operated using standard 5 V USB sources that power mobile devices (via battery, solar, or hand crank action). Time-resolved fluorescence detection and quantification is achieved using a smartphone camera and integrated image analysis app. Standard sample preparation is enabled by leveraging the drone's motors as centrifuges via 3D printed snap-on attachments. These advancements make it possible to build a complete DNA/RNA analysis system at a cost of $50 ($US). Our instrument is rugged and versatile, enabling pinpoint deployment of sophisticated diagnostics to distributed field sites. This capability is demonstrated by successful in-flight replication of Staphylococcus aureus and -phage DNA targets in under 20 min. The ability to perform rapid in-flight assays with smartphone connectivity eliminates delays between sample collection and analysis so that test results can be delivered in minutes, suggesting new possibilities for drone-based systems to function in broader and more sophisticated roles beyond cargo transport and imaging.

published proceedings

  • Anal Chem

altmetric score

  • 41.1

author list (cited authors)

  • Priye, A., Wong, S., Bi, Y., Carpio, M., Chang, J., Coen, M., ... Ugaz, V. M.

citation count

  • 108

complete list of authors

  • Priye, Aashish||Wong, Season||Bi, Yuanpeng||Carpio, Miguel||Chang, Jamison||Coen, Mauricio||Cope, Danielle||Harris, Jacob||Johnson, James||Keller, Alexandra||Lim, Richard||Lu, Stanley||Millard, Alex||Pangelinan, Adriano||Patel, Neal||Smith, Luke||Chan, Kamfai||Ugaz, Victor M

publication date

  • May 2016