Particle velocity measurements with macroscopic fluorescence imaging in lymph tissue mimicking microfluidic phantoms Conference Paper uri icon


  • Ultrasound poroelastography can quantify structural and mechanical properties of tissues such as stiffness, compressibility, and fluid flow rate. This novel ultrasound technique is being explored to detect tissue changes associated with lymphatic disease. We have constructed a macroscopic fluorescence imaging system to validate ultrasonic fluid flow measurements and to provide high resolution imaging of microfluidic phantoms. The optical imaging system is composed of a white light source, excitation and emission filters, and a camera with a zoom lens. The field of view can be adjusted from 100 mm x 75 mm to 10 mm x 7.5 mm. The microfluidic device is made of polydimethylsiloxane (PDMS) and has 9 channels, each 40 m deep with widths ranging from 30 m to 200 m. A syringe pump was used to propel water containing 15 m diameter fluorescent microspheres through the microchannels, with flow rates ranging from 0.5 l/min to 10 l/min. Video was captured at a rate of 25 frames/sec. The velocity of the microspheres in the microchannels was calculated using an algorithm that tracked the movement of the fluorescent microspheres. The imaging system was able to measure particle velocities ranging from 0.2 mm/sec to 10 mm/sec. The range of flow velocities of interest in lymph vessels is between 1 mm/sec to 10 mm/sec; therefore our imaging system is sufficient to measure particle velocity in phantoms modeling lymphatic flow. 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).

name of conference

  • Multimodal Biomedical Imaging VI

published proceedings


author list (cited authors)

  • Hennessy, R., Koo, C., Phuc, T., Han, A., Righetti, R., & Maitland, K. C.

citation count

  • 2

complete list of authors

  • Hennessy, Ricky||Koo, Chiwan||Phuc, Ton||Han, Arum||Righetti, Raffaella||Maitland, Kristen C

editor list (cited editors)

  • Azar, F. S., & Intes, X.

publication date

  • January 2011