Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres. Academic Article

abstract

• We have developed a 3D coaxial flow-focusing nozzle device for the mass production of monodispersed collagen microspheres and chemically crosslinked them using EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide) and N-hydroxysuccinimide (NHS). The size of the microspheres was varied between 200 m and 600 m by adjusting the ratio of the flow rates of the dispersed and continuous phases. MDA231-GFP cells were attached to the surface of these particles and their viability was investigated. Because they are comprised of a natural biomaterial, these collagen microspheres will have numerous applications, including bone regeneration scaffolds for tissue engineering and analyses of cancer cell interactions in a 3D environment.

authors

• Kameoka, Jun

published proceedings

• Annu Int Conf IEEE Eng Med Biol Soc

author list (cited authors)

• Jaligama, S., Po-Jung Huang, .., & Kameoka, J.

citation count

• 2

complete list of authors

• Jaligama, Sravani||Kameoka, Jun

publication date

• August 2016

publisher

• Institute of Electrical and Electronics Engineers (IEEE)  Publisher

published in

• Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference  Journal

keywords

• Biocompatible Materials
• Bone Regeneration
• Cell Line, Tumor
• Collagen
• Cross-Linking Reagents
• Humans
• Microscopy, Electron, Scanning
• Microspheres
• Succinimides
• Tissue Engineering
• Tissue Scaffolds

PubMed Central ID

• 28269214

Digital Object Identifier (DOI)

• 10.1109/EMBC.2016.7591658

start page

• 4220

end page

• 4223

volume

• 2016

URL

• http%3A%2F%2Fdx.doi.org%2F10.1109%2Fembc.2016.7591658