Selection of reference genes for expression analysis in the potato psyllid, Bactericera cockerelli. Academic Article

abstract

• The selection of reference genes is a crucial step for quantitative real-time PCR analyses and increasingly the use of more than one reference gene for accurate and reliable normalization is being recommended. In this study, a set of six genes was selected and their stability was assessed in different life stages and female organs of Bactericera cockerelli harbouring or not the bacterial pathogen 'Candidatus Liberibacter solanacearum' (Lso) haplotype B. The stability of each gene was determined using the BestKeeper, NormFinder and GeNorm programs. These analyses identified elongation factor-1a, ribosomal protein subunit L5 and ribosomal protein subunit 18 as the most stable genes to analyse gene expression during the insect life stages irrespective of Lso presence; Lso haplotype B only affected their respective ranking. By contrast, a common set of normalizers could not be found amongst the different female organs tested (bacteriomes, alimentary canals and reproductive organs).

authors

• Tamborindeguy, Cecilia

published proceedings

• Insect Mol Biol

author list (cited authors)

• Ibanez, F., & Tamborindeguy, C.

citation count

• 23

complete list of authors

• Ibanez, F||Tamborindeguy, C

publication date

• June 2016

publisher

• Wiley  Publisher

published in

• INSECT MOLECULAR BIOLOGY  Journal

keywords

• Animals
• Bactericera Cockerelli
• Female
• Gene Expression
• Gene Expression Profiling
• Genes, Insect
• Hemiptera
• Liberibacter
• Life Cycle Stages
• Nucleic Acid Amplification Techniques
• Polymerase Chain Reaction
• Psyllids
• Reference Genes
• Reference Standards

PubMed Central ID

• 26936438

Digital Object Identifier (DOI)

• 10.1111/imb.12219

start page

• 227

end page

• 238

volume

• 25

issue

• 3

URL

• http%3A%2F%2Fdx.doi.org%2F10.1111%2Fimb.12219