Transcriptional State of the Mouse Mammary Tumor Virus Promoter Can Affect Topological Domain Size in Vivo * Academic Article uri icon


  • Unrestrained DNA supercoiling and the number of topological domains were measured within a 1.8 megabase pair chromosomal region consisting of about 200 tandem repeats of a mouse mammary tumor virus promoter-driven ha-v-ras gene. When uninduced, unrestrained negative supercoiling was organized into 32-kilobase pair (kb) topological domains. Upon induction, DNA supercoiling throughout the region was completely relaxed. Supercoiling was detected, however, when elongation was blocked before or following induction. The formation of transcription initiation complexes upon addition of dexamethasone decreased the domain size to 16 kb. During transcription the domain size was 9 kb, the length of one repeat. These results suggest that topological domain boundaries can be "functional" in nature, being established by the formation of activated and elongating transcription complexes.

author list (cited authors)

  • Kramer, P. R., Fragoso, G., Pennie, W., Htun, H., Hager, G. L., & Sinden, R. R.

citation count

  • 44

complete list of authors

  • Kramer, PR||Fragoso, G||Pennie, W||Htun, H||Hager, GL||Sinden, RR

publication date

  • October 1999