17-Beta-estradiol regulates expression of genes that function in macrophage activation and cholesterol homeostasis. Academic Article

abstract

• Macrophage activation and cholesterol processing can be affected by changes in estrogen concentrations. However, there is a paucity of information about the genes and mechanisms regulating this estrogen effect. In primary monocyte-derived macrophages we detected transcript and protein for estrogen receptor beta (ERbeta). Determination of genes regulated by estrogen was completed using cDNA arrays and semiquantitative RT-PCR on RNA isolated from macrophages cultured in serum free media containing (5-10) x 10(-9)M 17-beta-estradiol and subsequently deprived of estrogen for a 24h period. The data indicate that the transcript levels of interleukin 1 receptor antagonist (IL-1ra), beta 2-microglobulin, annexin XI and the LXR(alpha) receptor significantly increased and that Ly-GDI transcript levels significantly decreased after estrogen withdrawal; data congruent with estrogen depletion regulating macrophage inflammatory and biochemical processes. Treatment of THP-1 cells with phorbol 12-myristate-13-acetate in the presence or absence of estrogen indicate that differentiation to a macrophage-like cell type was a prerequisite for production of the estrogen response. In addition, experiments using cycloheximide treatment, that blocks nascent protein synthesis, indicated that estrogen withdrawal affected the transcript levels of LXR(alpha) and IL-1ra through dissimilar pathways.

authors

• Kramer, Phillip

published proceedings

• J Steroid Biochem Mol Biol

author list (cited authors)

• Kramer, P. R., & Wray, S.

citation count

• 57

complete list of authors

• Kramer, PR||Wray, S

publication date

• January 2002

publisher

• Elsevier  Publisher

published in

• Journal of Steroid Biochemistry and Molecular Biology  Journal

keywords

• Blotting, Western
• Cell Differentiation
• Cells, Cultured
• Cholesterol
• Cycloheximide
• DNA, Complementary
• Estradiol
• Estrogen Receptor Alpha
• Estrogen Receptor Beta
• Estrogens
• Female
• Gene Expression Regulation
• Humans
• Immunohistochemistry
• Macrophage Activation
• Macrophages
• Molecular Sequence Data
• Monocytes
• Oligonucleotide Array Sequence Analysis
• Protein Binding
• Protein Synthesis Inhibitors
• RNA
• RNA, Messenger
• Receptors, Estrogen
• Reverse Transcriptase Polymerase Chain Reaction
• Tamoxifen
• Tetradecanoylphorbol Acetate
• Time Factors

Digital Object Identifier (DOI)

• 10.1016/s0960-0760(02)00065-1

start page

• 203

end page

• 216

volume

• 81

issue

• 3

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs0960-0760%2802%2900065-1