Protein domains and residues involved in the CheZ/CheAS interaction.
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abstract
CheZ localizes to chemoreceptor patches by binding CheA-short (CheA(S)). Residues 70 to 134 of CheZ, constituting the apical loops and part of the dimerization domain, suffice for localization. Replacements of Tyr-118, Ile-119, Leu-123, Arg-124, and Leu-126 of CheA interfere with localization. These residues are exposed in the 'P1 domain of CheA(S).