Relationships Between Follicular Size and Antral Fluid Steroid Concentrations at Three Stages of the Estrous Cycle in the Ewe Academic Article uri icon

abstract

  • This study examined the relationship between follicular size and antral fluid (AF) concentrations of estradiol, testosterone, and progesterone at three stages of the sheep estrous cycle. Ewes were ovariectomized (OVX) during the luteal phase (Group 1), and either prior to (Group 2), or after the peak (Group 3) of the preovulatory LH surge. In Experiment 1, with slaughterhouse material used as a random source of follicles at all stages of the estrous cycle, follicular diameter and AF volume were found to be highly significantly correlated; in Experiment 2, follicular size was estimated by measuring AF volume. Follicles from OVX ewes were grouped based upon follicular size: small follicles (<5.3 mm in diameter) contained <40 μl of AF, and large follicles (>5.3 mm in diameter) contained >40 μl of AF. AF concentrations of progesterone and estradiol were significantly correlated with AF volume in large follicles in Groups 2 and 3; however, no correlation was seen in small follicles. AF testosterone concentrations were inversely related to antral fluid volume in small follicles in Group 1 and in large follicles in Group 2. AF progesterone concentrations were low whereas AF testosterone concentrations were elevated in small follicles in Group 2, and in large follicles AF testosterone and estradiol concentrations were significantly elevated. In conclusion, in follicles with >40 μl AF volume, follicular size may be used as a reliable indicator of AF steroid concentrations, but only during the follicular phase of the estrous cycle. These data are indicative of rapidly changing AF concentrations of estradiol, testosterone, and progesterone during the follicular phase and imply that changes in follicular steroidogenesis are reflected in AF steroid concentrations. Furthermore, small follicles appear to have a limited capability to synthesize progesterone in Group 2 animals, and small follicles at all stages of the estrous cycle have limited aromatase activity.

author list (cited authors)

  • England, B. G., Dahmer, M. K., & Webb, R.

citation count

  • 15

publication date

  • June 1981

keywords

  • Animals
  • Body Fluids
  • Estradiol
  • Estrus
  • Female
  • Ovarian Follicle
  • Pregnancy
  • Progesterone
  • Sheep
  • Testosterone