Enzyme-linked immunosorbent assay for ovine interferon-tau. Academic Article

abstract

• Ovine interferon-tau (OvIFN-tau) is a trophectoderm secretory protein responsible for maintenance of corpus luteum function during early pregnancy. Methods for its quantitation include an antiviral assay and radioimmunoassay (RIA), both of which have disadvantages. We therefore developed an ELISA for OvIFN-tau that is specific, rapid, and 40-fold more sensitive than the current RIA. It uses a monoclonal antibody (Ab) HL129, which binds both native and recombinant (r) OvIFN-tau. The ELISA accurately detected known amounts of rOvIFN-tau added to yeast and tissue culture medium and to sheep serum at concentrations between 0.005 and 50,000 ng/ml. Inter- and intraassay coefficients of variation were 9.3 +/- 0.8 and 6.1 +/- 1.4%, respectively. Data are given for the reliability and reproducibility of the ELISA for measuring native OvIFN-tau and rOvIFN-tau.

authors

• Bazer, Fuller

published proceedings

• J Interferon Cytokine Res

altmetric score

• 3

author list (cited authors)

• Zhu, D., Ott, T. L., & Bazer, F. W.

citation count

• 8

complete list of authors

• Zhu, D||Ott, TL||Bazer, FW

publication date

• February 1996

publisher

• Mary Ann Liebert  Publisher

published in

• Journal of Interferon and Cytokine Research  Journal

keywords

• Animals
• Cost Control
• Enzyme-Linked Immunosorbent Assay
• Female
• Interferon Type I
• Linear Models
• Pregnancy
• Pregnancy Proteins
• Radioimmunoassay
• Reproducibility Of Results
• Sensitivity And Specificity
• Sheep
• Time Factors
• Vesicular Stomatitis Indiana Virus

PubMed Central ID

• 8742367

Digital Object Identifier (DOI)

• 10.1089/jir.1996.16.147

start page

• 147

end page

• 150

volume

• 16

issue

• 2

URL

• http://dx.doi.org/10.1089/jir.1996.16.147