Automatic acquisition of fiducial markers and alignment of images in tilt series for electron tomography.

abstract

• Three-dimensional reconstruction of a section of biological tissue by electron tomography requires precise alignment of a series of two-dimensional images of the section made at numerous successive tilt angles. Gold beads on or in the section serve as fiducial markers. A scheme is described that automatically detects the position of these markers and indexes them from image to image. The resulting set of position vectors are arranged in a matrix representation of the tilt geometry and, by inversion, alignment information is obtained. The scheme is convenient, requires little operator time and provides an accuracy of < 2 pixels RMS. A tilt series of 60-70 images can be aligned in approximately 30 min on any modern desktop computer.

authors

• Mcmahan, Uel

published proceedings

• J Electron Microsc (Tokyo)

altmetric score

• 3

author list (cited authors)

• Ress, D., Harlow, M. L., Schwarz, M., Marshall, R. M., & McMahan, U. J.

citation count

• 65

complete list of authors

• Ress, D||Harlow, ML||Schwarz, M||Marshall, RM||McMahan, UJ

publication date

• January 1999

publisher

• Oxford University Press (OUP)  Publisher

published in

• Journal of Electron Microscopy  Journal

keywords

• Algorithms
• Animals
• Gold
• Image Processing, Computer-Assisted
• Microscopy, Electron
• Microspheres
• Muscle, Skeletal
• Neuromuscular Junction
• Rana Pipiens
• Tomography

PubMed Central ID

• 10425746

Digital Object Identifier (DOI)

• 10.1093/oxfordjournals.jmicro.a023679

start page

• 277

end page

• 287

volume

• 48

issue

• 3

URL

• http%3A%2F%2Fdx.doi.org%2F10.1093%2Foxfordjournals.jmicro.a023679