A post-transcriptional mechanism contributes to circadian cycling of a per-beta-galactosidase fusion protein. Academic Article

abstract

• The period gene (per) of Drosophila melanogaster affects circadian rhythms. Circadian fluctuations in per mRNA levels are thought to contribute to circadian fluctuations in per protein levels in the heads of adult flies. To address the mechanisms underlying these oscillatory phenomena, we have analyzed RNA and protein cycling from two per-beta-galactosidase fusion genes. These studies demonstrate that 5' noncoding sequences from per are sufficient to cause the fusion mRNA levels to cycle in a wild-type (rhythmic) background. Protein cycling requires additional sequences derived from the per coding region. The data suggest that there is a per-dependent posttranscriptional mechanism that is under circadian clock control required for per protein levels to fluctuate in a rhythmic fashion.

authors

• Hardin, Paul

published proceedings

• Proc Natl Acad Sci U S A

author list (cited authors)

• Zwiebel, L. J., Hardin, P. E., Liu, X., Hall, J. C., & Rosbash, M.

citation count

• 49

complete list of authors

• Zwiebel, LJ||Hardin, PE||Liu, X||Hall, JC||Rosbash, M

publication date

• May 1991

publisher

• Proceedings of the National Academy of Sciences  Publisher

published in

• Proceedings of the National Academy of Sciences of the United States of America  Journal

keywords

• Animals
• Circadian Rhythm
• Cloning, Molecular
• Drosophila Melanogaster
• Drosophila Proteins
• Gene Expression Regulation
• Nuclear Proteins
• Period Circadian Proteins
• Periodicity
• Proteins
• RNA, Messenger
• Recombinant Fusion Proteins
• Restriction Mapping
• Transcription, Genetic
• Transformation, Genetic

PubMed Central ID

• 1902573

Digital Object Identifier (DOI)

• 10.1073/pnas.88.9.3882

start page

• 3882

end page

• 3886

volume

• 88

issue

• 9

URL

• http%3A%2F%2Fdx.doi.org%2F10.1073%2Fpnas.88.9.3882