Interlocked feedback loops within the Drosophila circadian oscillator. Academic Article uri icon

abstract

  • Drosophila Clock (dClk) is rhythmically expressed, with peaks in mRNA and protein (dCLK) abundance early in the morning. dClk mRNA cycling is shown here to be regulated by PERIOD-TIMELESS (PER-TIM)-mediated release of dCLK- and CYCLE (CYC)-dependent repression. Lack of both PER-TIM derepression and dCLK-CYC repression results in high levels of dClk mRNA, which implies that a separate dClk activator is present. These results demonstrate that the Drosophila circadian feedback loop is composed of two interlocked negative feedback loops: a per-tim loop, which is activated by dCLK-CYC and repressed by PER-TIM, and a dClk loop, which is repressed by dCLK-CYC and derepressed by PER-TIM.

published proceedings

  • Science

altmetric score

  • 3

author list (cited authors)

  • Glossop, N. R., Lyons, L. C., & Hardin, P. E.

citation count

  • 300

complete list of authors

  • Glossop, NR||Lyons, LC||Hardin, PE

publication date

  • October 1999