Specific sequences outside the E-box are required for proper per expression and behavioral rescue. uri icon

abstract

  • A 69 bp circadian regulatory sequence (CRS) upstream of the per gene is sufficient to drive circadian transcription, mediate proper spatial expression, and rescue behavioral rhythmicity in per01 flies. Within the CRS, an E-box is required for transcriptional activation by two basic-helix-loop-helix (bHLH) PERARNT-SIM (PAS) transcription factors, dCLOCK (dCLK) and CYCLE (CYC). To define sequences within the CRS that are required for spatial expression, circadian expression, and behavioral rhythmicity, a series of mutants that alter blocks of 3 to 12 nucleotides across the entire CRS were used to drive lacZ or per expression in vivo. As expected, the E-box within the CRS is necessary for high-level expression and behavioral rhythmicity, but sequences outside the E-box are also required for transcriptional activation, proper spatial expression, and behavioral rhythmicity. These results indicate that the dCLK-CYC target site extends beyond the E-box and that factors other than dCLK and CYC modulate per transcription.

published proceedings

  • J Biol Rhythms

author list (cited authors)

  • Lyons, L. C., Darlington, T. K., Hao, H., Houl, J., Kay, S. A., & Hardin, P. E.

citation count

  • 26

complete list of authors

  • Lyons, LC||Darlington, TK||Hao, H||Houl, J||Kay, SA||Hardin, PE

publication date

  • December 2000