Circadian oscillations in period gene mRNA levels are transcriptionally regulated. Academic Article

abstract

• The period (per) gene is involved in regulating circadian rhythms in Drosophila melanogaster. The per gene is expressed in a circadian manner, where fluctuations in per mRNA abundance are influenced by its own translation product, which also cycles in abundance. Since per gene expression is necessary for circadian rhythmicity, we sought to determine how certain features of this feedback loop operate. The results of this study reveal that fluctuations in per mRNA are primarily controlled by fluctuations in per gene transcription, that per mRNA has a relatively short half-life, and that sequences sufficient to drive per mRNA cycling are present in 1.3 kilobases of 5' flanking sequences. These and other results indicate that the per feedback loop has all of the basic properties necessary to be a component of a circadian oscillator.

authors

• Hardin, Paul

published proceedings

• Proc Natl Acad Sci U S A

altmetric score

• 3

author list (cited authors)

• Hardin, P. E., Hall, J. C., & Rosbash, M.

citation count

• 185

complete list of authors

• Hardin, PE||Hall, JC||Rosbash, M

publication date

• December 1992

publisher

• Proceedings of the National Academy of Sciences  Publisher

published in

• Proceedings of the National Academy of Sciences of the United States of America  Journal

keywords

• Animals
• Circadian Rhythm
• Drosophila Melanogaster
• Drosophila Proteins
• Feedback
• Gene Expression Regulation
• Genes, Insect
• Nuclear Proteins
• Nucleic Acid Precursors
• Period Circadian Proteins
• Promoter Regions, Genetic
• Proteins
• RNA, Messenger
• Transcription, Genetic

PubMed Central ID

• 1465387

Digital Object Identifier (DOI)

• 10.1073/pnas.89.24.11711

start page

• 11711

end page

• 11715

volume

• 89

issue

• 24

URL

• http%3A%2F%2Fdx.doi.org%2F10.1073%2Fpnas.89.24.11711