Confocal epipolarization microscopy of gold probes in plant cells and protoplasts. Academic Article uri icon


  • This chapter describes the cellular distribution of colloidal gold imaging in three dimensions at the light level using confocal laser scanning microscopy (CLSM) in the epipolarization modeconfocal laser scanning epipolarization microscopy (CLSEM). The conventional use of CLSM in biological samples has been for fluorescence analysis. Confocal laser scanning fluorescence microscopy (CLSFM) gives much sharper images because out-of focus fluoresced light, scatter, and flare are reduced. By providing sharp optical sections in the z dimension, a better understanding of the three-dimensional (3D) organization of the structure is possible. Although suggestions have been published concerning the possibility of using epipolarization optics in confocal laser scanning microscopes, it has only recently been reported. The chapter discusses three applications of CLSEM: (1) fluid phase uptake of gold colloids by plant protoplasts, (2) analysis of ligand-gold binding to plant cells and protoplasts, and (3) determination of cytoplasmic and wall delivery of biolistic gold beads. The major advantage of CLSEM is the use of reflection instead of fluorescence. Reflectance analysis of planar objects provides intrinsically higher signal and axial resolution over fluorescence analysis. 1995, Academic Press Inc.

published proceedings

  • Methods Cell Biol

author list (cited authors)

  • Griffing, L. R., Villanueva, M. A., Taylor, J., & Moon, S.

citation count

  • 3

complete list of authors

  • Griffing, LR||Villanueva, MA||Taylor, J||Moon, S

publication date

  • January 1995