A cell-density sensing factor regulates the lifetime of a chemoattractant-induced G alpha-GTP conformation. Academic Article

abstract

• Starving Dictyostelium discoideum cells monitor the local density of other starving cells by simultaneously secreting and sensing CMF. CMF regulates signal transduction through the chemoattractant cAMP receptor, cAR1. cAR1 activates a heterotrimeric G protein by stimulating G alpha 2 to release GDP and bind GTP. We show here that the rate of cAMP-stimulated GTP hydrolysis in membranes from cells exposed to CMF is roughly 4 times slower than in membranes from untreated cells, even though the rate of GTP binding is the same. This hydrolysis is abolished in cells lacking G alpha 2. Our data thus suggest that CMF regulates cAMP signal transduction in part by prolonging the lifetime of the G alpha 2-GTP complex.

authors

• Gomer, Richard

published proceedings

• FEBS Lett

author list (cited authors)

• Brazill, D. T., Gundersen, R., & Gomer, R. H.

citation count

• 12

complete list of authors

• Brazill, DT||Gundersen, R||Gomer, RH

publication date

• March 1997

publisher

• Wiley  Publisher

published in

• FEBS Letters  Journal

keywords

• Animals
• Cell Adhesion Molecules
• Chemokines
• Cyclic AMP
• Dictyostelium
• GTP-Binding Proteins
• Guanosine Triphosphate
• Hydrolysis
• Protein Conformation
• Protozoan Proteins
• Signal Transduction

PubMed Central ID

• 9074646

Digital Object Identifier (DOI)

• 10.1016/s0014-5793(97)00104-x

start page

• 100

end page

• 104

volume

• 404

issue

• 1