A novel method for oral stimulant administration in the neonate rat and similar species. Academic Article

abstract

• Sixty male and female Long-Evans hooded rats were administered 1, 2, or 5mg/kg methylphenidate (MPH) suspended in apple juice on postnatal day (P)15 or P40 using a novel, non-invasive oral administration technique. Plasma was collected 15 min after ingestion and analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS) to confirm appropriate concentrations. HPLC-MS plasma analysis showed levels comparable to previous gavage studies using MPH. We have used this method successfully in subsequent behavioral studies as well. Since therapeutic MPH in humans is typically administered orally, oral dosing methods that have been verified in the rodent model are of value. We recommend employment of this alternative oral dosing technique as it is minimally invasive, can be used anytime during postnatal development, and does not depend upon voluntary consumption.

authors

• Smith, Laura

published proceedings

• J Neurosci Methods

author list (cited authors)

• Wheeler, T. L., Eppolito, A. K., Smith, L. N., Huff, T. B., & Smith, R. F.

citation count

• 28

complete list of authors

• Wheeler, Tracey L||Eppolito, Amy K||Smith, Laura N||Huff, Thomas B||Smith, Robert F

publication date

• January 2007

publisher

• Elsevier  Publisher

published in

• Journal of Neuroscience Methods  Journal

keywords

• Administration, Oral
• Animals
• Animals, Newborn
• Animals, Suckling
• Attention Deficit Disorder With Hyperactivity
• Beverages
• Conditioning, Psychological
• Disease Models, Animal
• Female
• Male
• Malus
• Methylphenidate
• Rats
• Rats, Long-Evans
• Stress, Physiological
• Sympathomimetics

PubMed Central ID

• 16942798

Digital Object Identifier (DOI)

• 10.1016/j.jneumeth.2006.07.019

start page

• 282

end page

• 285

volume

• 159

issue

• 2

URL

• http://dx.doi.org/10.1016/j.jneumeth.2006.07.019