Elemental mapping and quantitative analysis of Cu, Zn, and Fe in rat brain sections by laser ablation ICP-MS. Academic Article

abstract

• This report details the application of laser ablation quadrupole ICP-MS for the (multi)elemental mapping of 100-microm-thick sections of rat brain. The laser spot size used was 60 microm, and the laser scan speed was 120 microm s(-1). The analysis was relatively rapid, allowing mapping of a whole brain thin section (approximately 1 cm2) in about 2 h. Furthermore, the method was amenable to multi-element data collection including the physiologically important elements P and S and afforded sub microg g(-1) detection limits for the important trace elements Cu and Zn. Calibrations were performed with pressed pellets of biological certified reference materials, and the elemental distributions and concentrations of Cu, Zn, and Fe were determined in whole rat brain sections. The distributions and concentration ranges for these elements were consistent with previous studies and demonstrate the utility of this technique for rapid mapping of brain thin sections.

authors

• Smith, Laura

published proceedings

• Anal Bioanal Chem

author list (cited authors)

• Jackson, B., Harper, S., Smith, L., & Flinn, J.

citation count

• 102

complete list of authors

• Jackson, Brian||Harper, Steve||Smith, Laura||Flinn, Jane

publication date

• February 2006

publisher

• Springer Nature  Publisher

published in

• Analytical and Bioanalytical Chemistry  Journal

keywords

• Animals
• Brain Chemistry
• Calibration
• Copper
• Iron
• Lasers
• Male
• Mass Spectrometry
• Rats
• Rats, Sprague-Dawley
• Reference Standards
• Zinc

PubMed Central ID

• 16402172

Digital Object Identifier (DOI)

• 10.1007/s00216-005-0264-6

start page

• 951

end page

• 957

volume

• 384

issue

• 4

URL

• http://dx.doi.org/10.1007/s00216-005-0264-6