Acoustophoretic droplet sorting system for high-throughput algal mutant library screening
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14CBMS. Microalgae have the potential to sustainably produce liquid biofuel. Developing algal strains with faster growth rate through mutagenesis is one of the major routes in making algal biofuel economically feasible. Droplet microfluidic systems can provide sufficiently high throughput to screen large algal mutant library. Current methods of droplet sorting such as hydrodynamic and dielectrophoretic sorting rely on fluorescent detection. Here we present an acoustophoretic droplet sorting system that is capable of selecting fast-growing strains from an algal mutant library. The principle of separation is based on the difference in acoustophoretic forces exerted to droplets having different number of cells inside the droplet, where the number of cells is an indicator of the growth rate of the initial single mutant cell encapsulated in the droplets.