Synchrony of division in mass cultures of Paramecium tetraurelia by a selective density-labelling technique. Academic Article

abstract

• SUMMARYA technique has been developed for achieving synchrony of division by selection for immediate pre- and post-fission cells of Paramecium tetraurelia. Cell populations are fed granular tantalum, followed by density-gradient centrifugation. Dividing cells, which do not feed, are separated from feeding interphase cells. The selected cells are division-synchronous for at least two generations when resuspended in fresh culture fluid. This technique provides a rapid means of obtaining large numbers of synchronously dividing paramecia for cell-cycle studies and has general application for selective enrichment of non-feeding sub-populations of cells.

authors

• Aufderheide, Karl

published proceedings

• Genet Res

author list (cited authors)

• Aufderheide, K.

citation count

• 1

complete list of authors

• Aufderheide, K

publication date

• April 1976

publisher

• Hindawi  Publisher

published in

• Genetical Research  Journal

keywords

• Animals
• Methods
• Mitosis
• Paramecium
• Stimulation, Chemical
• Tantalum

PubMed Central ID

• 1278681

Digital Object Identifier (DOI)

• 10.1017/s0016672300016372

start page

• 171

end page

• 177

volume

• 27

issue

• 2

URL

• http://dx.doi.org/10.1017/s0016672300016372