Unpaired genes do not silence their paired neighbors. Academic Article

abstract

• During meiotic chromosome pairing, a loop of unpaired DNA induces the silencing of all paired and unpaired homologous DNA via meiotic silencing, an RNA-mediated post-transcriptional gene-silencing mechanism. To test the effect of unpaired DNA on adjacent genes, we constructed strains containing the DNA of a transformation marker integrated immediately downstream of the Ascospore maturation-1 ( Asm-1) gene and tested whether this unpaired DNA silences asm-1(+). We conclude that unpaired downstream DNA has no effect on Asm-1 expression during meiosis or ascospore development, which suggests that the silencing signal produced by unpaired DNA does not propagate onto adjacent paired regions.

authors

• Aramayo, Rodolfo

published proceedings

• Curr Genet

author list (cited authors)

• Kutil, B. L., Seong, K., & Aramayo, R.

citation count

• 11

complete list of authors

• Kutil, Brandi L||Seong, Kye-Yong||Aramayo, Rodolfo

publication date

• September 2003

publisher

• Springer Nature  Publisher

published in

• Current Genetics  Journal

keywords

• Chromosome Pairing
• Gene Expression Regulation, Fungal
• Gene Silencing
• Genes, Reporter
• Genetic Vectors
• Neurospora
• Plasmids

PubMed Central ID

• 12802506

Digital Object Identifier (DOI)

• 10.1007/s00294-003-0412-5

start page

• 425

end page

• 432

volume

• 43

issue

• 6

URL

• http://dx.doi.org/10.1007/s00294-003-0412-5